Fig. 7
From: The Elk1/MMP-9 axis regulates E-cadherin and occludin in ventilator-induced lung injury
si-Elk1 or MMP-9 ameliorated the expressions of E-cadherin, occludin, Elk1, and MMP-9. A Representative Western blotting of E-cadherin, occludin, Elk1, MMP-9, and TIMP-1 in MLE-12 cells. B–F The density of the proteins at the control group was used as a standard. G Ratio of MMP-9/TIMP-1. *P < 0.05, vs. the control group. **P < 0.05, vs. the MV(4 h) group. ***P > 0.05, vs. the MV(4 h) group. H: Immunofluorescence was used to detected the distribution of occludin (green) and E-cadherin (red), and the nuclei were stained with DAPI (blue) in C57BL/6 treated with si-Elk1 or si-MMP-9 and high tidal volume for 4 h. All experiments were repeated at five times