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Fig. 2 | Respiratory Research

Fig. 2

From: Proteome of airway surface liquid and mucus in newborn wildtype and cystic fibrosis piglets

Fig. 2

Airway gel-forming mucins were found in fraction 5, the mucus fraction. A Normalized intensities of MUC5AC in all fractions, unstimulated (open circles, n = 4) and stimulated (filled circles, n = 6) WT piglets. Red filled circles in fraction 4 and 5 indicate the normalized intensity in the same piglet in fraction 4 (the highest value) and fraction 5. B Normalized intensities of MUC5B in all fractions, unstimulated (open circles, n = 4) and stimulated (filled circles, n = 6) WT piglets. Green filled circles in fraction 4 and 5 indicate the normalized intensity in the same piglet in fraction 4 (the highest value) and fraction 5. C Immunostaining of paraffin sections from newborn WT piglets demonstrating that MUC5AC (red) was produced by surface epithelial goblet cells (left image) and MUC5B (green) was mainly produced by submucosal glands (right image). Scale bar left image 50 µm, scale bar right image 20 µm. D Comparison between normalized intensities for MUC5AC in fraction 4 and 5 for unstimulated (open circles, n = 4) and stimulated (filled circles, n = 6) WT piglets. There was more MUC5AC in unstimulated samples from fraction 5 than fraction 4, *P = 0.03, Mann–Whitney test. Dark red filled circle indicates outlier. E Comparison between levels of MUC5B in fraction 4 and 5 for unstimulated (open circles, n = 4) and stimulated (filled circles, n = 6) WT piglets. There was more MUC5B in unstimulated samples from fraction 5 than fraction 4, *P = 0.03, Mann–Whitney test. Dark green filled circle indicates outlier. F Pooling normalized intensities from unstimulated and stimulated (10 µM carbachol) WT piglets (n = 10) and CF piglets (n = 6) respectively demonstrated that there was more MUC5AC than MUC5B in fraction 5. G There was more MUC5AC (red open circles, n = 4, 16 values) than MUC5B (green open circles, n = 4, 16 values) in unstimulated WT piglets, pooled relative intensities from fraction 1, 2, 3 and 4. The same was found for stimulated WT piglets, pooled relative intensities from fraction 1, 2, 3 and 4 (MUC5AC red closed circles, n = 6, 24 values, MUC5B green closed circles, n = 6, 24 values). Each data point in AC, EG represents one piglet and results are presented with median and interquartile range. Scale bar in C 50 µm

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