Fig. 1

Expression levels of FASN were upregulated in lung endothelial cells under obesity or metabolic stress in response to LPS. a Body weight of lean and DIO mice during regular chow diet and high-fat diet feeding, respectively; n = 83 in the lean mice group and n = 125 in the DIO mice group. Student’s t test analysis. b, c Representative H&E images of the lungs (b) and quantitative analysis of the lung injury (c) in the lean and DIO mice 8 h after LPS or saline intratracheal administration; n = 6 mice in each group. Scale bar: 100 μm. One-way ANOVA and LSD posthoc analysis. d, e Representative western blots (d) and quantitative analysis (e) of FASN expression in freshly isolated lung endothelial cells from the lean and DIO mice 6 h after LPS or saline intratracheal administration; n = 10–13 in each group. One-way ANOVA and LSD posthoc analysis. f–i Representative western blots (f, h) and quantitative analysis (g, i) of FASN expression in each group of mouse lung endothelial cells (MLECs). n = 6 biologically independent experiments. One-way ANOVA and LSD or Games–Howell posthoc analysis. Data were represented as mean ± SD