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Fig. 3 | Respiratory Research

Fig. 3

From: Inhibition of fatty acid synthase protects obese mice from acute lung injury via ameliorating lung endothelial dysfunction

Fig. 3

FASN regulated the levels of mitochondrial ROS in MLECs under metabolic stress in response to LPS. a–f Representative western blots of Mfn2, p-Drp1 and Drp1 (a, d), and corresponding quantitative analysis of Mfn2 (b, e) and p-Drp1/Drp (c, f) in each group of MLECs. n = 3 biologically independent experiments. One-way ANOVA and LSD or Games–Howell posthoc analysis. g–j Representative immunofluorescence images (g), flow cytometry analysis (h) and quantitative analysis (i, j) of mitoSOX in each group of MLECs. n = 3 biologically independent experiments. Scale bar: 100 μm. One-way ANOVA and Games–Howell posthoc analysis. k FASN activity were detected in MLECs under PA in response to LPS with or without C75 treatment. One-way ANOVA and LSD posthoc analysis. n = 3 biologically independent experiments. Data were represented as mean ± SD

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