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Fig. 9 | Respiratory Research

Fig. 9

From: Inhibition of fatty acid synthase protects obese mice from acute lung injury via ameliorating lung endothelial dysfunction

Fig. 9

C75 significantly increased the expression levels of VE-cadherin in lung endothelial cells from DIO mice. a, b Representative western blots (a) and quantitative analysis (b) of VE-cadherin expression in freshly isolated lung endothelial cells from the lean and DIO mice at 6 h after LPS or saline intratracheal administration. n = 10–13 mice in each group. One-way ANOVA and Games–Howell posthoc analysis. c, d HE images of lungs (c) and assessment of the lung injury (d) in LPS-treated DIO mice with or without C75 pretreatment. n = 5–6 mice in each group. Scale bar: 100 μm. Student’s t test analysis. e–k The lean and DIO mice were intratracheally instilled LPS or saline for 8 h. C75 was intraperitoneally administered in DIO mice 30 min before LPS instillation. The FASN activity of lung tissues were detected in each group of mice (e). Representative western blots and quantitative analysis of FASN in lung tissues (f, g), and FASN (h, i) and VE-cadherin (j, k) in freshly isolated lung endothelial cells in each group of mice. n = 6 mice in each group. One-way ANOVA and LSD or Games–Howell posthoc analysis. Data were represented as mean ± SD

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