Figure 4
From: Hypoxia regulates human lung fibroblast proliferation via p53-dependent and -independent pathways
Stability of p21 mRNA exposed to hypoxia in NHLF. Cultured NHLF were exposed to 0.1% or 2% oxygen, or 100 μM DFX, and the stability of p21 mRNA was measured using RT-PCR after addition of 400 nM Act D. The stability of p21 mRNA significantly decreased after 8 and 24 h of moderate hypoxia relative to the 21% oxygen control. Data are percentages of each maximal mRNA expression. 21% oxygen control (open bars), 2% oxygen (solid bars), 0.1% oxygen (shaded bars), and DFX (diagonal bars). Data are expressed as mean ± SE (n = 6). *P < 0.05 versus the 21% oxygen control.