Figure 6

Effects of RGDS on phosphorylation of ERK1/2 (A, B), JNK1/2(C, D) and p38 MAP kinase (E, F) in lung tissue. Where indicated, mice were administered RGDS or RGES (5 mg/kg, i.p.) once 1 h before LPS treatment and sacrificed 4 h post-LPS. Mice were also administered these peptides (5 mg/kg, i.p.) once 1 h before or 2 h after LPS and sacrificed 24 h post-LPS. Western blotting was performed with each anti-specific (phospho) Ab on lung tissue homogenates. Relative values for phosphorylated MAP kinase versus MAP kinase, respectively, are indicated below the gel. Values represent means ± SEM of 5 mice per group. *Significantly different from saline treated controls, p < 0.05; ⁺significantly different from animals treated with LPS only, p < 0.05.