Figure 4

EGR1 and CYR61 protein levels in lung tissue following injurious ventilation. Lung tissue sections stained for EGR1 and CYR61 proteins using immunohistochemical techniques. The lung tissue sections shown are representative of the sections from unventilated age-matched control fetuses and preterm lambs at 2 hours after a 15 minute period of injurious ventilation (IV). The brown stain represents lung tissue containing the EGR1 or CYR61 protein. Slides incubated without the primary or secondary antibodies did not show any evidence of brown staining (data not shown).