Figure 6
Right ventricular hypertrophy is depicted as the increase in the ratio RV/(LV+IVS) compared to the room air control (panel A) and ventricular wall thickness, indicated as the RV/LV ratio (panel B) in room air-exposed controls (RA, white bars), age-matched O 2 -exposed controls (O 2 , black bars) and sildenafil-treated rat pups (100 mg/kg/day [Sil 100 ], gray bars) under hyperoxia on day 10. Cardiac characteristics are presented in table 2. Paraffin sections of the right ventricular wall stained with polyclonal tenascin C (panels C-E) of room-air (RA, panel C) and O2-exposed controls (panel D), and age-matched pups treated with sildenafil (100 mg/kg/day) under hyperoxia (panel E) at 10 days of age. Note the extravascular expression of tenascin C in the right ventricle in oxygen-exposed pups (panel D) and the absence of staining after treatment with sildenafil (panel E) and in room air controls (panel C). Pictures were taken at a 400× magnification.