Figure 4

Effect of AG on HSP47, TGFβ1, p-Smad2, Smad2, p-Smad3 and Smad3 protein after bleomycin instillation. Western blot was performed to determine the expression levels of target proteins. (A) Representative blotting images of HSP47, TGFβ1, p-Smad2, Smad2, p-Smad3, Smad3, and β-actin were shown. Densitometric analysis of HSP47 (B), TGFβ1 (C), p-Smad2 (D), p-Smad3 (E) protein expression relative to the β-actin control was presented. Data represent mean ± SD, n = 5 in each group, * p < 0.01 vs SA group; † p < 0.05 vs BLM group; ‡ p < 0.01 vs BLM group.