Figure 3

Comparison of Methylation mapping of DAPK promoter in exhaled breath and mouthwash-exfoliated DNA. (a): Methylation mapping of exhaled breath DNA. (b) Methylation mapping of mouthwash-exfoliated DNA. Exhaled breath condensate (EBC) from 37 of 38 initially recruited consecutive donors and available mouthwash from 36 of the 37 EBC donors, was screened using the tBGS multiplex technique for simultaneous assay of three gene promoters' CpG islands within ~200-300 bp surrounding the TIS. Only mapping results for the DAPK promoter are shown. Subject historical smoking features are listed on the left. Mean percent of sites methylated is listed by smoking and case strata, in larger font, on the right. Wide inter-individual methylation variability within any given smoking stratum is apparent. All samples are collected prior to any diagnostic or therapeutic procedure.