Table 1 Primer sequences used for real time PCR amplification
From: Different regulation of cigarette smoke induced inflammation in upper versus lower airways
Forward primer (5'→ 3') | Reverse primer (5'→ 3') | TaqMan probe (5'-6-FAM → TAMRA-3') | Amplicon size | Genbank Accession number | |
|---|---|---|---|---|---|
ACTB | AGAGGGAAATCGTGCGTGAC | CAATAGTGATGACCTGGCCGT | CACTGCCGCATCCTCTTCCTCCC | 139 | NM_007393 |
GCP-2 | GCTGCCCCTTCCTCAGTCAT | CACCGTAGGGCACTGTGGA | 129 | NM_009141 | |
MCP-1 | CTTCTGGGCCTGCTGTTCA | CCAGCCTACTCATTGGGATCA | CTCAGCCAGATGCAGTTAACGCCCC | 126 | NM_011 333 |
MIP-3α | CCAGGCAGAAGCAAGCAACT | TCGGCCATCTGTCTTGTGAA | TGTTGCCTCTCGTACATACAGACGCCA | 71 | AJ222694 1 |
TGF-β1 | TGACGTCACTGGAGTTGTACGG | GGTTCATGTCATGGATGGTGC | TTCAGCGCTCACTGCTCTTGTGACAG | 170 | M13177 |