Figure 4
From: Cigarette smoke and lipopolysaccharide induce a proliferative airway smooth muscle phenotype
CSE and LPS increase cyclin D1 expression. Serum-deprived BTSM cells were treated with CSE (15%) for 1 h, or LPS (1 μg/ml) or PDGF (30 ng/ml) for 24 h. Cell lysates were obtained 24 h after stimulation and analyzed by immunoblotting for cyclin D1 and β-actin to correct for protein loading. Cyclin D1 was quantified using densitometry and normalized to control expression. Data represent means ± S.E.M. of 4-7 experiments. *P < 0.05 vs control