Figure 7
Proximal pulmonary vascular cell proliferating and migrating capacity. (A) Mitogenic activity of proximal PASMC isolated from lung donors (n = 4), non-thromboembolic PH (n = 4) and CTEPH patients (n = 4). Subconfluent cells were starved for 24 h in 0.2% FBS medium. Mitogenic activity was measured in the presence of 0.2% (control) or 10% FBS plus 0.5 μCi.mL-1 of [3H]-thymidine for 36 h. ANOVA, p = 0.02; *p < 0.05. (B) Migrating capacity of proximal PASMC isolated from lung donors (n = 3), non-thromboembolic PH (n = 3) and CTEPH patients (n = 3). Subconfluent cells were starved for 24 h in 0.2% FBS medium. PASMC migration was evaluated using a scratch wound assay in the presence of 0.2% (control) or 10% FBS for 36 hours. ANOVA, p = 0.02; *p < 0.02. (C) Mitogenic activity of proximal PAEC isolated from lung donor (n = 1), non-thromboembolic PH (n = 3) and CTEPH patients (n = 4). Subconfluent cells were starved for 24 h in 0.2% FBS medium. Mitogenic activity was measured in the presence of 0.2% (control) or 5% FBS as described above. ANOVA, p = 0.0002, *p < 0.02, **p < 0.002. Open symbols, 0.2% FBS; plain symbols, 10%. PH group: circle, PAH; triangle, non PAH-PH. (A, B) or 5% (C) FBS. For each patient, 1 to 3 independent experiments were performed in triplicate. Regarding EC from the lung donor, 3 independent experiments were performed in triplicate.