Figure 1

Demonstration of HLA-G in primary dAEC grown in ALI culture. A. Expression of HLA-G as demonstrated by qPCR. One sample from each subject was tested in triplicate and normalized to 18S-RNA expression. A positive control cell line (JEG-3) analyzed at the same time is shown for reference. B. Protein expression of HLA-G as demonstrated by western blot. Whole cell lysates collected from six different dAEC cultures were resolved using the 4H84 mAb that binds both, and cannot distinguish between, the soluble G5 and membrane bound G1 isoforms. A single band at ~ 39 kD consistent with HLA-G is demonstrated. A negative control, the JAR choriocarcinoma cell line that does not express HLA-G, and a positive control, the JEG3 choriocarcinoma cell line, are shown.