Figure 2

Hyperoxia-dependent inhibition of lung morphogenesis is attenuated by VEGF164 and VEGF188. E10.5 lung explants were harvested, infected with Ad-DsRed (A,B), Ad-Vegf164 (C), or Ad-Vegf188 (D) and then exposed at E13 to either 3% (A) or 50% (B-D) oxygen for 2 d. (E) Total number of terminal branches and (F) total branch length were quantitated in explants treated as above. n =4 for each group, *p <0.05 vs. 3% alone and **p <0.05 vs. 50% oxygen + DsRed at E12 + 2. Photographs at 4x magnification. Bar = 1 mm.