Figure 1

Satellite cell detection performed by immunostaining. (A) Vastus lateralis muscle cryosections were immunostained for co-expression and localization of nuclear Paired box transcription factor 7 (Pax7, green), DAPI (blue) and laminin (red). Labeled nuclei located deep to the basal lamina and positive for pax7 were counted as satellite cells (white arrow). (B) Labeled nuclei located deep to the basal lamina and positive for Pax7 were counted as satellite cells. The number of satellite cells is expressed over 100 fibers and reported as a ratio; ANOVA; p > 0.05. Ratios are calculated from 9 healthy subjects, 11 COPD (MTCSA > 70 cm²) and 6 COPD (MTCSA < 70 cm²).