Figure 4
Effect of ATP and ACh on cultured rat tracheal myocytes. A: percentage of cells responding to 10-3 M ATP, and amplitude of the [Ca2+]i peak, in cells cultured for 72 h in non-proliferating medium (black columns, n = 27) and in cells cultured for 10 days in proliferating medium (open columns, n = 35). B: typical single [Ca2+]i recording of a cell cultured for 10 days in proliferating medium stimulated with 10-3 M ATP. C: typical single [Ca2+]i response to 10-5 M ACh in tracheal myocytes freshly isolated (J0) (n = 61) and cultured for 48 h in non-proliferating medium (n = 26). D: percentage of cells responding to 10-5 M ACh, and amplitude of the [Ca2+]i peak, in freshly isolated myocytes (black columns, n = 61) and in cells cultured for 48 h in non-proliferating medium (open columns, n = 26). *P < 0.05 versus responses in freshly isolated cells.