Figure 1

Anti-Muc2 monoclonal antibody (C4) binds to Muc2 apomucin in ELISA. Guinea pig Muc2 apomucin fragment (10, 50, 100, and 200 ng/well) purified from transformed bacterial culture lysate was measured by ELISA using purified C4 monoclonal antibody (400 μg/ml), diluted 1:100 in 0.3% cold-water fish gelatin. Results indicate the C4 monoclonal antibody binds to the Muc2 apomucin fragment in a concentration-dependent manner. Absorbance values read at 450 nm are shown corrected for background. Each sample was measured in triplicate, and data are shown as mean ± SEM. This assay was repeated three times with similar results.