Figure 1From: Toll-like receptors in cellular subsets of human tonsil T cells: altered expression during recurrent tonsillitisThe isolated CD4+and CD8+T cells are highly purified. Tonsils were minced and T cells were separated from other mononuclear cells using SRBC rosetting followed by density gradient centrifugation. CD4+ and CD8+ T cells were further isolated by negative selection using magnetic beads. The isolation procedure was accompanied by FACS analysis of surface phenotype (purity > 95%).Back to article page