Figure 6

Role of p65 in regulating anti-apoptotic protein level in response to cigarette smoke exposure. Panel A: effect of cigarette smoke on the levels of anti-apoptotic and pro-apoptotic proteins. HBECs were treated with varying concentrations of CSE for 24 hours. Cell lysates were subjected for immunoblotting of Bcl-XL, Bad, Bax, XIAP, Bcl2 and β-actin as described in the methods. Panel B: Role p65 in regulating Bcl-XL synthesis in response to cigarette smoke exposure. HBECs were transfected with control siRNA or p65 siRNA followed by exposing to cigarette smoke. Cell lysates were obtained 24 hours after CSE exposure and subjected for immunoblotting for Bcl-XL and β-actin as described in the methods. Data presented is one representative from 2 separate experiments.