Figure 1

Baseline and IL-1β-stimulated prostaglandin production. IPF lung fibroblasts (HF-IPF) (n = 6) and normal human lung fibroblasts (HF-NL) (n = 5) were incubated with or without IL-1β (2.5 ng/ml) for 24 hours. At this time culture media was replaced with media containing arachidonic acid (30 μM) and fibroblasts were incubated for 30 min. A profile of COX-2-dependent eicosanoid products (PGE₂ [a], TXB₂ [b], PGF_2α [c], and 6-κ-PGF_1α [d]) was measured from the supernatant by gas chromatography/mass spectrometry and expressed as median production in ng/10⁶ cells/30 min, with their respective range included in parenthesis.