Figure 9

Role of caspase 8 the expression of IL-1 family. Male C57bl/6 mice were exposed to vehicle (aerosolised saline) or LPS (1 mg/ml) for 30 minutes. Lung tissue was collected at different time after the exposure and caspase 8 activity measured (A). Data are represented as mean ± S.E.M. for n = 6 animals in each group. Wild type, age matched control mice and GM mice (mice missing functional ASC and IPAF) were exposed to vehicle (aerosolised saline) or LPS (1 mg/ml) for 30 minutes. Lung tissue was collected at different time after the exposure and caspase 8 activity measured (B). Data are represented as mean ± S.E.M. for n = 6 animals in each group. Wild type, age matched control mice and GM mice (mice missing functional IL-1β, IL-18 and caspase 1/11) were exposed to vehicle (aerosolised saline) or LPS (1 mg/ml) for 30 minutes. BALF was collected 6 hours after the exposure and IL-1α (C) measured by ELISA. Data are represented as mean ± S.E.M. for n = 8 animals in each group. Statistical significance was determined using Mann–Whitney U test. # = P < 0.05, denoting a significant difference between the LPS exposed and vehicle exposed wild-type groups.