Figure 4

Representative macro and microphotographs of SW mouse lungs 24 h after intra-tracheal challenge with ouabain (0.075 μmol/animal), GLP (12 μg of GLP protein/animal) or ouabain plus GLP. Macroscopic photos of mouse lungs challenged with (A) sterile saline (control), (B) ouabain or (C) GLP. Photomicrographs of the same lungs, showing intact alveolar structures in the control group (D) and hemorrhagic sites in the lungs from mice challenged with leptospiral GLP (E), ouabain (F) or GLP plus ouabain (GLP + ouabain) (G). Plethysmographic analysis of lung function (H), where the enhanced pause (Penh) was used to represent airway resistance. The plethysmographic results are represented as the mean ± SEM of 7 to 15 animals. *P < 0.05.