Figure 6

SARM-TRIF signaling pathway is involved in regulating MMP-12 expression. Nude mice were inoculated with RSV or mock infected with the culture supernatant. Resveratrol or PBS was injected intraperitoneally for 5 days consecutively. SARM siRNA 3–1 and the negative siRNA vector were transfected intranasally. The disease parameters were assessed at day 5 post-RSV infection. Nude mice were divided into five groups: control: mock-infected and PBS treated mice group; RSV: RSV-infected and PBS treated mice group; RSV + RES: RSV-infected and resveratrol treated mice group; RSV + RES + 3-1: RSV-infected, resveratrol treated and SARM siRNA 3–1 transfected mice group; RSV + RES + Negative: RSV-infected, resveratrol treated and negative siRNA vector administrated mice group. A: Inflammatory cells infiltrating into BALF. B: AHR in response to increasing doses of methacholine. C: Western blotting analysis of the expression of SARM and TRIF in the lung tissues. D: Semi-quantified expression of SARM and TRIF normalized to β-actin. E: MMP-12 protein levels in BALF. Data are representative of two independent experiments performed on 12 animals per group. In graph B, ***, p < 0.001 shown comparing the RSV group to the RSV + RES group; ^^^, p < 0.001 shown comparing the RSV + RES + 3-1 group to the RSV + RES + Negative group. In the other graphs, *, p < 0.05, **, p < 0.01, ***, p < 0.001 shown comparing the corresponding mice groups are connected by a line.