Figure 2From: Treatment with a sphingosine analog after the inception of house dust mite-induced airway inflammation alleviates key features of experimental asthma Experimental design. Mice were instilled i.n. with HDM or saline (SAL) from day 1 to 10, along which time they were also treated i.t. with vehicle (dH2O) or dH2O containing 2.5 μg of AAL-R from day 7 to day 10, one hour before HDM administration. In a subset of experiments, 3.5 μg of AFD-R (molar equivalent of AAL-R) were used instead of AAL-R. 24 h after the last exposure, mice were either euthanized for the collection of BALF and tissue sampling or tested to assess their degree of airway responsiveness to MCh.Back to article page