Figure 4

Denatonium reduces mitochondrial membrane potential. A) The TEM microphotographs show the ultrastructure of control cells (a1,a2) and denatonium (2 mM, 72 h) treated A549 cells (b1, b2, b3). Swollen mitochondria (***) were observed in treated cells (b2, b3). B) A549 cells were stained with the cationic lipophilic dye, JC-1 and analyzed by FACS after treatment with denatonium for 72 h. C) 16HBE cells were stained with the cationic lipophilic dye JC-1 and analyzed by FACS after treatment with denatonium for 24 h. The figures are representative profiles of at least three experiments. The histogram shows the quantification of mitochondrial membrane potential. (**p < 0.01, ***p < 0.001).