Figure 3

Inhibition of ROS production (A) and vimentin induction (B and C) by oral administration of 10 mg/kg astragalin in OVA-challenged mouse airway tissues. ROS production was measured by using the fluorescent stain DCF-DA and quantified at λ = 485 nm excitation and λ = 538 nm emission (A). Tissue extracts were subject to Western blot analysis with anti-vimentin (B). The bar graphs (mean ± SEM, n = 3) represent quantitative results of the upper bands obtained from a densitometer. Means without a common letter differ, P < 0.05. Vimentin identified as green staining was visualized with a FITC-conjugated secondary antibody and nuclear staining was done with blue dye 4′,6-diamidino-2-phenylindole (C). Each photograph is representative of four mice. Magnification: 200-fold.