Fig. 5
From: Anti-inflammatory potential of PI3Kδ and JAK inhibitors in asthma patients
Effects of dexamethasone, PIK-294 and tofacinib on TCR-induced pSTAT5 activation in lymphocytes. Isolated blood T-cells from asthma patients (n = 5) and healthy subjects (n = 6) and BAL cells (n = 4 asthma plus n = 3 healthy) were treated with 1000nM dexamethasone, PIK-294 or tofacinib for 1 h before TCR-stimulation for 4 h. Levels of phosphorylated STAT5 in CD3+ cells were quantified by flow cytometry. Data is presented as mean % pSTAT5 positive CD3 cells +/− standard deviation. Drug effects were assessed by 1-way ANOVA with a Dunnett’s multiple comparison test against the stimulated no drug control: *p < 0.05