Fig. 5

Effects of ANP on TGF-β signaling in mouse endothelial cells. a Western blot analysis with the antibodies indicated at left using the cell lysates prepared from SVEC/GC-A cells stimulated with TGF-β (1 ng/ml) and/or ANP (0.1 μM) for 30 min. The blot shown is representative of three independent experiments. b–d Quantitative RT-PCR analysis of MCP-1 (b), collagen 1A (c), and CTGF (d) in SVEC/GC-A stimulated with TGF-β (1 ng/ml) and/or ANP (0.1 μM) for 4 h. Relative mRNA expressions in vehicle, ANP, TGF-β/vehicle, and TGF-β/ANP groups are shown. Values represent means ± SEMs (n = 6 per group). *p < 0.05