Fig. 3

Caspase-3 activation during injury resolution after a single instillation of gastric fluid. a Representative Western blot of lung tissue homogenate illustrating the time course of changes in cleaved caspase-3 expression. This immunoblot is from a single set of animals (one animal sample per time point) and is representative of 6 separate sets. The immunoblot shows that the 19/17 kDa bands seen in the control sample show no change at day 4, a significant enlargement at day 7 and are not different from the control bands at day 14. β-tubulin immunoblot shows equal protein loading. b Densitometric analysis of six independent Western blots expressed as times over control, showing a significant 60% increase in cleaved caspase-3 at day 7 only. Western blot bands were normalized to beta-tubulin. Data are means ± SD. **: p < 0.01 with respect to controls