Fig. 1

VEGF promotes MUC5AC expression at both mRNA and protein level. PBECs were incubated with indicated doses of VEGF for 9 h, and then real-time PCR performed. The values are normalized relative to the GAPDH standard (A). PBECs were incubated at indicated times of VEGF (50 ng/ml), and then real-time PCR performed (B). PBECs were incubated with indicated doses of VEGF for 24 h (C). PBECs were incubated at indicated times of VEGF (50 ng/ml), and then western blotting analysis for MUC5AC was performed. β-actin was used as a loading control (D). All data are representative of three independent experiments. The blots were quantified by densitometry. Values represent the means ± SEM. ^*P < 0.05, ^**P < 0.005 vs. control; n = 3