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Fig. 2 | Respiratory Research

Fig. 2

From: Resveratrol attenuates cigarette smoke induced endothelial apoptosis by activating Notch1 signaling mediated autophagy

Fig. 2

RESV attenuated apoptosis and induced autophagy in CSE treated HUVECs. To investigate the role of RESV in CSE induced apoptosis, HUVECs were pretreated with 40 μM RESV for 2 h, followed by cotreatment with 2.5% CSE for 24 h. Autophagy related proteins (LC3b-I/II, Beclin1, p62, mTOR) and apoptotic protein (cleaved caspase3) were detected by Western blot. β-Actin was used as loading control (a). Bar charts show the quantification of LC3B-II/LC3b-I, Beclin1, P62, mTOR and cleaved caspase3. Values are presented as means ± SD (b, c, d, e, f). Cell apoptosis was detected by flow cytometry (g). Statistical analysis was calculated (h). *P < 0.05, **P < 0.01 compared between the marked groups

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Respiratory Research

ISSN: 1465-993X

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