Table 1 Primer sequences for quantitative real-time polymerase chain reaction
Gene | Primer sequence (5′ → 3′) |
|---|---|
Notch1 | F: CGCCTTTGTGCTTCTGTTCTT |
R: TCCCGCCGCTTCTTCTT | |
Notch2 | F: ATATTGATGACTGCCCTAACCAC |
R: ATCTCCACTCCAGCCGTTG | |
Notch4 | F: CCCATTAAAAGGCAGGCTGGA |
R: CACGTGGAAGATGTCTGCTCT | |
Jag1 | F: TACTGTGGGACTCATCAGCCGTGT |
R: CAGCAATTTCACAGTTGGGTCCT | |
Jag2 | F: TCGTACTTGCACTCACAATACCA |
R: CACACTCGCAGCGGAACCA | |
Dll1 | F: CCAGCCCTTGTAAGAACGGA |
R: ACAGATTTTGCCGTAGAAGCC | |
Dll4 | F: ATGCCTGTGCCTCGAGTCCC |
R: CCAGCCCCACACCCAGCGAGA | |
β-actin | F: ACCCTGAAGTACCCCATCGAG |
R: AGCACAGCCTGGATAGCAAC |