Longitudinal analysis of sinus microbiota post endoscopic surgery in patients with cystic fibrosis and chronic rhinosinusitis: a pilot study

Wagner Mackenzie, Brett; Dassi, Camila; Vivekanandan, Anitha; Zoing, Melissa; Douglas, Richard G.; Biswas, Kristi

doi:10.1186/s12931-021-01697-w

Table 2 Patient sputum and nasal swab cultivation report alongside bacterial 16S rRNA gene sequencing results

From: Longitudinal analysis of sinus microbiota post endoscopic surgery in patients with cystic fibrosis and chronic rhinosinusitis: a pilot study

Patient	Collection timepoint^b	Sputum sample	Bacterial 16S rRNA sequencing	Nasal swab	Antimicrobial resistance
P01	Baseline	Staphylococcus aureus Other^a	Enterobacteriaceae, Enterobacter, Staphylococcus, Corynebacterium	Other	None assessed
	6	Staphylococcus aureus, Morganella morganii, Enterobacter cloacae Other	Enterobacteriaceae, Enterobacter, Staphylococcus	Enterobacter cloacae Other	Amoxycillin, Amoxycillin + clavulanate, cefuroxime
	13	Staphylococcus aureus, Chryseobacterium indologenes Other	Enterobacteriaceae, Enterobacter, Staphylococcus, Corynebacterium	Staphylococcus aureus Other	Penicillin
	15	Staphylococcus aureus, mould Other	Enterobacteriaceae, Enterobacter, Staphylococcus	Enterobacter cloacae, Staphylococcus aureus	Amoxycillin, cefuroxime, amoxycillin + clavulanate, penicillin
	18	Staphylococcus aureus, mould Other	Enterobacteriaceae, Enterobacter, Staphylococcus	Staphylococcus aureus, Enterobacter cloacae	Penicillin, erythromycin, amoxycillin, cefuroxime, amoxycillin + clavulanate
P02	Baseline	Pseudomonas aeruginosa Other	Staphylococcus, Pseudomonas	Pseudomonas aeruginosa Other	Gentamicin
	2	Pseudomonas aeruginosa Other	Staphylococcus, Pseudomonas	Other	–
	6	–	Pseudomonas	–	–
	11	Pseudomonas aeruginosa Other	Pseudomonas, Corynebacterium	Pseudomonas aeruginosa	Gentamicin
P03	Baseline	Staphylococcus aureus	Staphylococcus	Staphylococcus aureus	Penicillin, flucloxacillin
	3	–	Staphylococcus, Corynebacterium	–	–
	6	Staphylococcus aureus Other	Staphylococcus, Enterobacter	Staphylococcus aureus	Penicillin, flucloxacillin
	12	Staphylococcus aureus Other	Staphylococcus, Enterobacteriaceae	Staphylococcus aureus	Penicillin, flucloxacillin
P04	Baseline	Data not collected^c	Pseudomonas, Staphylococcus	Pseudomonas aeruginosa, Aspergillus fumigatus Other	Gentamicin, amikacin, ciprofloxacin
	3	–	Pseudomonas	Pseudomonas aeruginosa	Gentamicin, ciprofloxacin
	6	–	Pseudomonas	Pseudomonas aeruginosa Other	Ceftazidime, gentamicin
	7	–	Pseudomonas, Staphylococcus, Corynebacterium	Pseudomonas aeruginosa, Fusarium solani complex Other	Gentamicin, amikacin, ciprofloxacin
	11		Pseudomonas, Staphylococcus	Pseudomonas aeruginosa Other	Gentamicin

In vitro antimicrobial resistance is noted only for nasal swab isolates. Results from left and right middle meatus swabs for molecular techniques are combined. In vitro antimicrobial resistance profiles from nasal swab bacteria are noted
^a‘Other’ refers to growth of bacterial colonies that were not considered clinically relevant. This includes mixed oropharyngeal bacteria, mixed Gram-positive and Gram-negative colonies, commensal flora or skin flora. These colonies were not further isolated or identified
^bCollection timepoint is given as months since baseline collection
^cSputum samples were not collected from this patient. Analysis of patient records revealed that prior to lung transplantation in 2012 the dominant organisms recovered from sputum were Pseudomonas aeruginosa and Aspergillus fumigatus. Sputum collected post lung transplantation, but prior to this study, cultured Pseudomonas aeruginosa

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ISSN: 1465-993X

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