Fig. 3
TGF-β1-induces protein upregulation of Tpm1.6/1.7 and Tpm2.1, but not Tpm3.1. Human lung fibroblasts were left untreated (control) or stimulated with TGF-β1 (2 ng/mL) for 0, 24 and 48 h. Tpm1.6/1.7 and Tpm2.1 protein upregulation was detected by Western blotting with the α/9d antibody, while Tpm3.1 was detected with the γ/9d antibody (compared to α-tubulin as a loading control). Results are shown as: a a representative Western blot; densitometric analysis of (b) Tpm2.1 and (c) Tpm1.6/1.7 protein upregulation (normalised with α-tubulin and expressed as fold increase compared to control at 0 h). Statistical analysis was performed using two-way ANOVA then Bonferroni's multiple comparisons test (where * denotes a significant effect of TGF-β1 on tropomyosin isoform upregulation (P < 0.05)). Data are mean ± SEM values from n = 7 human lung fibroblast primary cell cultures