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Fig. 7 | Respiratory Research

Fig. 7

From: TIPE2 inhibits PDGF-BB-induced phenotype switching in airway smooth muscle cells through the PI3K/Akt signaling pathway

Fig. 7

IGF-1 treatment reversed the effect of TIPE2 on ASMC phenotype switching. ASMCs were transfected with pcDNA3.0-TIPE2 for 48 h in the presence of IGF-1 (5 μM) and then treated with PDGF-BB. A The expression levels of PI3K and p-PI3K in ASMCs were determined by Western blotting. B The cell proliferation of ASMCs was measured by the MTT assay. C Cell migration of ASMCs was determined using the transwell assay. D The protein expression levels of calponin and SM22α in ASMCs were determined by Western blotting. E, F Quantification analysis of calponin and SM22α. Control group, *p < 0.05, vs. the pcDNA3.0 + PDGF-BB group, #p < 0.05, vs. the pcDNA3.0-TIPE2 + PDGF-BB group, &p < 0.05

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Respiratory Research

ISSN: 1465-993X

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