Fig. 5

Reduction of IL-8 by SC79 in A549 and primary airway cells in response to TNF-α or flagellin. a–d Cells were treated with TNF-α (0.1 μg/ml) ± SC79 (2.5 μg/ml) for 24 h before adding RNA lysis buffer followed by qPCR. Bars are expression relative to GAPDH (2^−ΔCT; mean ± SEM; n = 3). a ***p < 0.001 (CTL vs. TNF-α) and *p < 0.05 (TNF-α vs. TNF-α + SC). Raw ΔCT values were 6.99 ± 0.36 (control), 0.09 ± 0.3 (TNF-α; p < 0.01 vs control), and 1.95 ± 0.6 (TNF-α + SC79; p < 0.05 vs TNF-α alone). b **p < 0.01 (CTL vs. TNF-α) and *p < 0.05 (TNF-α vs. TNF-α + SC). Raw ΔCT values were 5.32 ± 0.25 (control), 3.24 ± 0.33 (TNF-α; p < 0.01 vs control), and 4.43 ± 0.32 (TNF-α + SC79; p < 0.05 vs TNF-α alone). c ***p < 0.001 (CTL vs. TNF-α) and ***p < 0.001 (TNF-α vs. TNF-α + SC). Raw ΔCT values were 4.47 ± 0.19 (control), 2.40 ± 0.18 (TNF-α; p < 0.01 vs control), and 4.70 ± 0.30 (TNF-α + SC79; p < 0.05 vs TNF-α alone). d **p < 0.01 (CTL vs. TNF-α) and *p < 0.05 (TNF-α vs. TNF-α + SC). Raw ΔCT values were 4.43 ± 0.17 (control), 2.04 ± 0.17 (TNF-α; p < 0.01 vs control), and 3.49 ± 0.21 (TNF-α + SC79; p < 0.05 vs TNF-α alone). All significance by one way ANOVA and Bonferroni posttest. e, f A549s and primary bronchial cells were treated with flagellin (1 μg/ml) ± SC79 (2.5 μg/ml) for 24 h and qPCR was performed. Bars are mean ± SEM; n = 3. e **p < 0.01 (CTL vs. flagellin) and *p < 0.05 (flagellin vs. flagellin + SC). Raw ΔCT values were 4.27 ± 0.26 (control), 0.72 ± 0.37 (flagellin; p < 0.01 vs control), and 2.53 ± 0.23 (flagellin + SC79; p < 0.05 vs flagellin only). f *p < 0.05 (CTL vs. flagellin) and ***p < 0.001 (flagellin vs. flagellin + SC). Raw ΔCT values were 5.07 ± 0.24 (control), 4.21 ± 0.34 (flagellin; p < 0.05 vs control), and 14 ± 0.57 (flagellin + SC79; p < 0.001 vs flagellin only). All significance by one-way ANOVA and Bonferroni posttest