Table 1 Human depletion effect
Sample ID | Treatment | Human DNA assay (Ct mean ± SD) | Human depletion [△Ct (CT meanUndepleted−CT meanDepleted)] | Human depletion (P value) | Bacteria DNA assay (Ct mean ± SD) | Bacteria concentration change [△Ct (CT meanUndepleted−CT meanDepleted)] | Bacteria concentration change (P value) |
|---|---|---|---|---|---|---|---|
S01 | Depleted Undepleted | 18.568 ± 0.160 18.059 ± 0.084 | −0.51 | 0.004 | 21.509 ± 0.055 20.186 ± 0.060 | −1.32 |  < 0.001 |
S02 | Depleted Undepleted | 19.629 ± 0.049 18.876 ± 0.138 | −0.82 | 0.005 | 20.693 ± 0.021 18.784 ± 0.128 | −1.91 | 0.001 |
S03 | Depleted Undepleted | 28.750 ± 0.202 18.843 ± 0.050 | −9.9 |  < 0.001 | 25.248 ± 0.211 23.190 ± .0111 | −2.05 |  < 0.001 |
S04 | Depleted Undepleted | 30.507 ± 0.511 19.077 ± 0.067 | −11.43 |  < 0.001 | 19.405 ± 0.169 19.323 ± 0.015 | −0.08 | 0.486 |
S05 | Depleted Undepleted | 24.546 ± 0.084 18.197 ± 0.386 | −6.35 |  < 0.001 | 19.693 ± 0.169 20.827 ± 0.308 | −1.13 | 0.017 |
S06 | Depleted Undepleted | 28.146 ± 1.059 18.495 ± 0.403 | −9.65 | 0.002 | 26.523 ± 0.144 20.589 ± 0.204 | −5.93 |  < 0.001 |
S08 | Depleted Undepleted | 19.629 ± 0.086 18.876 ± 0.179 | −0.75 | 0.001 | 21.538 ± 0.053 25.652 ± 0.222 | 4.11 |  < 0.001 |
S10 | Depleted Undepleted | 25.587 ± 0.093 18.903 ± 0.421 | −6.68 |  < 0.001 | 20.115 ± 0.286 19.610 ± 0.164 | −0.504 | 0.072 |
S12 | Depleted Undepleted | 27.051 ± 0.199 18.835 ± 0.129 | −8.21 |  < 0.001 | 24.641 ± 0.077 29.123 ± 0.185 | 4.48 |  < 0.001 |
S13 | Depleted Undepleted | 31.984 ± 1.372 18.505 ± 0.143 | −13.48 | 0.003 | 29.092 ± 0.055 22.696 ± 0.150 | −6.40 |  < 0.001 |
S14 | Depleted Undepleted | 23.710 ± 0.319 19.869 ± 0.131 | −3.84 |  < 0.001 | 13.605 ± 0.084 17.143 ± 0.067 | 3.53 |  < 0.001 |
S15 | Depleted Undepleted | 20.807 ± 0.838 19.466 ± 0.282 | −1.34 | 0.097 | 14.208 ± 0.175 16.705 ± 0.193 | 2.50 |  < 0.001 |