Accumulation of oxysterols in the erythrocytes of COVID-19 patients as a biomarker for case severity

Respiratory Research

Table 1 Calibration parameters of the acylcarnitines and oxysterols in the homogenate of erythrocytes

t_R, min	Name	Squared Regression coefficient, r²	Slope*	Intercept	Range, ng/μL	LOD, ng/μL
25.82	O-Palmitoleoyl-L-carnitine	0.9991	0.0425	0.0355	2.0–60	0.50
30.94	Palmitoyl-L-carnitine	0.9988	0.0399	0.0415	2.0–60	0.50
28.71	Linoleoyl-L-carnitine	0.9995	0.0517	0.0222	2.0–60	0.50
32.52	Oleoyl-L-carnitine	0.9999	0.0401	0.0066	2.0–60	0.50
37.64	Stearoyl-L-carnitine	0.9992	0.0452	0.0299	2.0–60	0.50
16.72	3-Hydroxyoleoylcarnitine	0.9987	0.0407	0.0425	2.0–60	0.50
32.55	Oleoyl-L-carnitine-d9, IS1	N/A	N/A	N/A	N/A	N/A
60.61	Cholesterol	0.9999	0.0417	0.0101	2.0–60	0.50
49.11	4,6-Cholestadien-3-one	0.9995	0.0398	0.0211	2.0–60	0.50
38.52	4β-Hydroxycholesterol-4-acetate	0.9997	0.0335	0.0198	2.0–60	0.50
38.82	4β-Hydroxycholesterol	0.9991	0.0392	0.0324	2.0–60	0.50
50.28	4-Cholestenone	0.9998	0.0378	0.0060	2.0–60	0.50
40.19	7-Ketocholesterol	0.9999	0.0414	0.0022	2.0–60	0.50
60.82	Cholesterol-d6, IS2	N/A	N/A	N/A	N/A	N/A
40.22	7-ketocholesterol-d7, IS3	N/A	N/A	N/A	N/A	N/A

^*The regression line was drawn by plotting the concentration (X independent variable), ng/μL, versus the peak area ratio of the analyte to the internal standard (Y dependent variable)

ISSN: 1465-993X