Fig. 2

Tobacco flavored e-cig inhibited fibroblast differentiation markers

HFL-1 cells exposed to air, PG/VG, and tobacco flavored e-cig for 10 min, and then cultured for 2 days. (A) cells were lysed and protein was isolated for western blotting, fibronectin, COL1A1, and αSMA were analyzed, GAPDH was used as the endogenous control. (B). RNA was isolated from cells, and FN1, COL1A1, and ACTA2 were measured by qRT-PCR, GAPDH was used as the endogenous control. (C) Cells were fixed, and stained with COL1A1, the fluorescence intensity was measured by EVOMS, fluorescence intensity /cell was calculated via ImageJ. Data presented as mean ± SEM. (n = 3–6. * P < 0.05, ** P < 0.01, *** P < 0.001 vs. air). Scale bar = 200 μm