Fig. 3

The measurement of FAPα in BALF is a marker of lung fibrogenesis: (A) Time-course of the concentration of FAPα in BALF day 3 (D3), day 10 (D10), day 16 (D16) and day 28 (D28) after instillation in bleomycin (black column bars) or 0,9% NaCl (control) mice (white column bars). Data are presented as mean with standard deviation and statistical analysis was performed using Two-Way ANOVA followed by Bonferroni correction test for multiple comparisons. Controls: n = 5–6; bleomcyin-treated mice: n = 6–8. (B) Effect of various doses of bleomycin (0,005, 0,01, 0,02 and 0,04U/mouse) on the FAPα BALF concentration at day 10 after bleomycin instillation. Data are presented as mean with standard deviation and statistical analysis was performed using One-Way ANOVA followed by Holm-Sidak’s post-hoc test. Controls: n = 24; 0.005U bleomycin-treated mice (B0.005), n = 13; 0.01U bleomycin-treated mice (B0.01), n = 13; 0.02U bleomycin-treated mice (B0.02), n = 14; 0.04U bleomycin-treated mice (B0.04), n = 7. C-F. Correlations between FAPα BALF concentration, the Ashcroft modified scale (C), the percentage of weight loss (expressed by the area under the curve (AUC)) (D), the concentration of hydroxyproline (OH-proline) (E) and the concentration of interleukin-6 (IL6) (F). The r corresponds to the Pearson coefficient for parametric correlation. *: p < 0.05; **: p < 0.01; ***: p < 0.001; ****: p < 0.0001