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Fig. 1 | Respiratory Research

Fig. 1

From: Circular RNA P4HB promotes glycolysis and tumor progression by binding with PKM2 in lung adenocarcinoma

Fig. 1

Circular RNA transcript of the P4HB gene. A Localization of circP4HB and back-splicing of exon 3 and exon 4. B Arrow showed the back-splicing site of circP4HB. C Agarose gel electrophoresis analysis of PCR products of divergent primer and convergent primer. ←  → divergent primers; →  ← convergent primers. GAPDH was used as a control. cDNA: complementary DNA, gDNA: genomic DNA. D Relative expression level of P4HB and circP4HB in A549 and SPC-A1 cells after RNase R digestion. DMSO treated cells were used as a control. E Copy number of P4HB in normal lung cells (16HBe) and lung cancer cell lines. HBE was used as a reference. F Expression of circP4HB in normal lung cells (16HBe) and lung cancer cell lines. HBE was used as a reference. (*p < 0.05, **p < 0.01, **p < 0.01, ****p < 0.0001, N.S no significance.)

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Respiratory Research

ISSN: 1465-993X

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