Fig. 1
From: Inhibition of PKA/CREB1 pathway confers sensitivity to ferroptosis in non-small cell lung cancer
PKA/CREB1 pathway is activated during ferroptosis. A Pan-cancer analysis of the Pearson correlation between the expression of CREB1 and activity of reactive oxygen species pathway. B, C Enrichment analysis demonstrated the top 10 enriched biological pathways between TCGA-LUAD (B) and LUSC (C) patients with high or low expression of CREB1. D The relative mRNA expression of CREB1 in A549 and H1299 cells when treated with DMSO, H89 (PKA inhibitor, 30 μM), or cAMP (PKA activator, 100 μM) for 48 h. E, F The protein level and corresponding quantification of PKA, phospho-PKA, CREB1, and phospho-CREB1 in A549 and H1299 cells (cropped blots) treated with DMSO, H89 (30 μM), cAMP (100 μM), RSL3 (ferroptosis inducer, 0.2 μM for A549, 0.05 μM for H1299), IKE (ferroptosis inducer, 2 μM), or their combinations for 48 h. TCGA, the Cancer Genome Atlas; LUAD, lung adenocarcinoma; LUSC, lung squamous cell carcinoma; H89, H-89 dihydrochloride; cAMP, 8-Bromo-cAMP sodium salt; IKE, Imidazole Ketone Erastin. Data were analyzed by Student’s t-test and presented by mean ± SD in triplicate. ns, not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001