Fig. 3
From: Inhibition of PKA/CREB1 pathway confers sensitivity to ferroptosis in non-small cell lung cancer
CREB1 plays a cardinal role in suppressing ferroptosis. A, B RT-qPCR (A) and immunoblotting (B, cropped blots) assays revealed the knockdown effect of three siRNAs targeting CREB1 in A549 cells. C Relative cell viability of A549 cells transfected with siNC (control siRNA) or siCREB1 treated with RSL3 in gradient concentrations. D, E RT-qPCR (D) and immunoblotting (E, cropped blots) assays revealed CREB1 mRNA expression in A549 and H1299 cells after transfection with NC (control shRNA), CREB1-SH1, or CREB1-SH2 lentivirus. F Relative cell viability of A549 and H1299 cells treated with RSL3 in gradient concentrations after transfection with NC, CREB1-SH1, or CREB1-SH2 lentivirus. G MDA production of A549 and H1299 cells after treatment with RSL3 (2 μM) for 8 h. H Relative cell viability of A549 and H1299 cells treated with DMSO plus RSL3 (2 μM), H89 (30 μM) plus RSL3 (2 μM), or cAMP (100 μM) plus RSL3 (2 μM) after transfection with NC, CREB1-SH1, or CREB1-SH2 lentivirus. I Lipid peroxidation measurement of different groups with or without pre-treatment of H89 (30 μM) or cAMP (100 μM) for 48 h. Data were analyzed by two-way ANOVA or Student’s t-test and presented by mean ± SD in triplicate. ns, not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001