Fig. 5
From: Inhibition of PKA/CREB1 pathway confers sensitivity to ferroptosis in non-small cell lung cancer
SCD reverses the effect of CREB1 knockdown on ferroptosis and CREB1 binds to the promoter region of SCD to promote SCD expression. A The histogram revealed the relative abundance of PE-PUFAs and PE-MUFAs in A549 cells transfected with NC or CREB1-SH1 lentivirus. B, C Cell toxicity assays (B) and lipid peroxidation measurement (C) demonstrated the rescue effect of SCD in CREB1-knockdown A549 and H1299 cells. D Predicted BS of CREB1 and design of primers for ChIP-qPCR. E ChIP-seq results demonstrated the CREB1-binding peak in the promoter region close to the TSS of SCD. F The enrichment of CREB1 in A549 and H1299 cells. G Dual-luciferase assays using luciferase plasmids containing WT or mutated type of the promoter region of SCD. PE, phosphatidylethanolamine; PUFAs, polyunsaturated fatty acids; MUFAs, monounsaturated fatty acids; BS, binding sites; ChIP, chromatin immunoprecipitation; TSS, transcription start site; WT, wild type. Data were analyzed by two-way ANOVA or Student’s t-test and presented by mean ± SD from three biological replicates. ns, not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001