Fig. 3
From: CSE triggers ferroptosis via SIRT4-mediated GNPAT deacetylation in the pathogenesis of COPD
Experimental validation of CSE-induced cell damage, inflammation and ROS in human alveolar epithelial cells. A549 cells were treated with DMSO, erastin (ferroptosis activator) or Fer-1 (ferroptosis inhibitor), accompanied by 5% CSE exposure. (A) Cell viability was detected by CCK-8 assay. (B) The LDH release was detected by LDH activity assays. (C) The levels of total ROS were determined using a 2’, 7’-dichlorofluorescein diacetate (DCFH-DA) kit. (D) Cell apoptosis was determined by flow cytometry assay. (E) Detection of lipid oxidation by the BODIPY 581/591 C11 probe method. Data are presented as the mean ± SD of three replicates and analyzed using ANOVA, followed by Tukey’s post-hoc test. ***p < 0.001, compared with blank; $p < 0.05, $$$p < 0.001, compared with 5% CSE; ###p < 0.001, compared with 5% CSE + DMSO