Fig. 4
From: CSE triggers ferroptosis via SIRT4-mediated GNPAT deacetylation in the pathogenesis of COPD
Experimental validation of CSE-induced markers associated with ferroptosis and plasmalogen biosynthesis. (A) Mitochondrial morphology associated with ferroptosis was determined by transmission electron microscopy. (B) Content detection of the ferroptosis-related markers MDA, GSH, and GPX4 by ELISA. (C) The mRNA expression of GNPAT was measured via quantitative real-time PCR. (D) The protein levels of GPX4 and GNPAT were detected by western blot analysis. (E) Immunofluorescence staining of GNPAT was displayed in different groups. Data are presented as the mean ± SD of three replicates and analyzed using ANOVA, followed by Tukey’s post-hoc test. *p < 0.05, ***p < 0.001, compared with blank; $p < 0.05, compared with 5% CSE; ##p < 0.01, ###p < 0.001, compared with 5% CSE + DMSO. The gels were cropped reasonably