Table 1 Primer pairs used for quantitative RT-PCR analysis
From: CSE triggers ferroptosis via SIRT4-mediated GNPAT deacetylation in the pathogenesis of COPD
Gene ID | Sequence (5’- 3’) |
|---|---|
H-GAPDH F | TGTTCGTCATGGGTGTGAAC |
H-GAPDH R | ATGGCATGGACTGTGGTCAT |
H-FAR-1Â F | CTCACCCAACCTAAACTGGCT |
H-FAR-1R | GCTTGCGATTACAGTAGGCATA |
H-AGPS F | TGAGTACCAATGAGTGCAAAGC |
H-AGPS R | GGTAAACCCATGCCACTAAGAG |
H-GNPAT F | AAGAGAGGAGGCATGTCAGT |
H-GNPAT R | AACCGAATGGCTCCAAGACG |
H-SIRT4 F | TCGTAGGCTGGCCTCAATTC |
H-SIRT4 R | CCCACAATCCAAGCACAGGA |
M-β-actin F | CATTGCTGACAGGATGCAGA |
M-β-actin R | CTGCTGGAAGGTGGACAGTGA |
M-FAR-1Â F | TAGTGGTCAACACGAGCCTTG |
M-FAR-1 R | GGCTTACAGCAATCCAGTAATGA |
M-AGPS F | AATGGATGGGGCTACAATGATTC |
M-AGPS R | TCCAGACTTACTCCAAGGGTG |
M-GNPAT F | GGAGCCATTCGGTTCTTTGC |
M-GNPAT R | AAGTCCATTCCTGCTGCGAT |