Fig. 1

TUFT1 was upregulated in lungs of IPF and BLM‑induced fibrotic mouse lungs. a Representative image of immunohistochemistry staining of TUFT1 in the lung sections from the Donor and IPF patient, n = 3 patients or donors, scale bars:100 µm. b Representative image of immunohistochemistry staining of Tuft1 in bleomycin induced fibrotic lung, n = 3 mouse per group, scale bars:100 µm. c The Tuft1 protein level in lung homogenates from bleomycin-induced fibrotic models was determined using Western blot analysis, n = 4 mouse per group. d Densitometry analysis of fig c (mean ± SD). e E-cadherin, N-cadherin, Vimentin and TUFT1 were determined by Western blot in A549 cells induced by TGF-β1, GAPDH used as loading control. f TUFT1, collagen I and α-SMA protein expression determined by Western blot in TGF-β1-induced MRC-5 cells, GAPDH used as loading control. g Densitometry analysis of fig f (mean ± SD). *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001